About Pregmate Ovulation Tests Identify the best time to conceive. Determine your most fertile days with Pregmate ovulation predictor kits. Maximize your chance of pregnancy with accurate ovulation prediction. Find your most fertile 12-48 hours with our discreet, simple and easy-to-use ovulation tests. Predict your fertility window. Get pregnant faster. Take control of your fertility. Pregmate ovulation tests accurately detect Luteinizing Hormone in urine. LH (Luteinizing Hormone) is one of the hormones produced by the pituitary gland. It is secreted at very low levels throughout your menstrual cycle. Once a developing egg follicle reaches a certain size, LH secretion surges to high levels. This hormone surge is what triggers ovulation about 24 to 48 hours later. Your LH surge is important because it initiates the beginning of ovulation. Ovulation is the release of a mature egg from the ovary. It signals the beginning of your fertile period. A positive result indicates the presence of a high amount of LH or LH surge. The amount of LH in your body will begin to decrease after ovulation, so you’ll only get a positive result during that crucial fertile period. Once the egg is released, it is only viable for about 24 hours. After that, your fertile window is over. This makes it very important to be able to identify this best time to conceive. Ovulation may be irregular because of conditions, emotions, and other factors in your life. You can’t presume that ovulation always occurs at the same time after menstruation. Therefore, you should test again in each menstrual cycle. When to begin testing? The length of the menstrual cycle is the duration from your first menstrual bleeding day to the day before the next bleeding begins. Determine the length of menstrual cycle before test. Please refer to the chart included in the package to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult a physician. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Test procedure: Immerse the strip into the urine. Take the strip out after 3 seconds. Lay the strip flat. Read results in 5 minutes. Reading the results: Positive (LH Surge): If two lines are visible and the test line is equal to or darker than the control line. Negative (No LH Surge): No test line or the test line is lighter than the control line.