Pregmate Ovulation Tests detect levels of Luteinizing Hormone (LH) in urine. Increase your chance of pregnancy with accurate ovulation prediction. Identify the best time to conceive. Know your most fertile days with Pregmate ovulation predictor kits. Predict your fertility window. Get pregnant faster. Luteinizing Hormone is one of the hormones produced by the pituitary gland. It is secreted at very low levels throughout your menstrual cycle. Once a developing egg follicle reaches a certain size, LH secretion surges to high levels. This hormone surge is what triggers ovulation about 24 to 48 hours later. Your LH surge is important because it initiates the beginning of ovulation. Measuring LH on a numeric basis, in addition to basic ovulation testing, may provide a better understanding of your LH levels and timing of LH surge, LH peak and ovulation window. How to do the test? Dip the strip into the urine for 3-5 seconds. Lay the strip flat. Read results in 5 minutes. How to read the results? Positive (LH Surge): Two lines are visible and the test line is equal to or darker than the control line. Negative (No LH Surge): No test line or the test line is lighter than the control line. Compare the Test Line to the lines on the color chart. The numbers on the chart represent the corresponding LH levels. Graph your result on the Fertility Chart and keep track of your LH curve from month to month. Measuring LH levels: Low: Test Line is lighter than line 25 on the color chart or test line is not present. High: If Test Line is similar to or darker than line 25 on the color chart, your LH is nearing a surge or surging. Most women at this concentration will ovulate within the next 48 hours. Peak: If Test Line is darker than line 40 on the color chart, your LH is nearing its highest levels.